Laufendes Projekt
"Personalausweis" für Mauersegler
Herstellung von Transponderringen zur individuellen Identifizierung und Nachverfolgung der Mauersegler
Langzeitstudie
AG Ökologie und Verhaltensbiologie
2022 |
Windfelder, A G; Müller, F H H; Larney, Mc B; Hentschel, M; Böhringer, A C; von Bredow, C R; Leinberger, F H; Kampschulte, M; Maier, L; von Bredow, Y M; Flocke, V; Merzendorfer, H; Krombach, G A; Vilcinskas, A; Grimm, J; Trenczek, T E; Flögel, U Nat Commun, 13 (1), S. 7216, 2022, ISSN: 2041-1723. @article{RN14335, title = {High-throughput screening of caterpillars as a platform to study host-microbe interactions and enteric immunity}, author = {A G Windfelder and F H H Müller and B Mc Larney and M Hentschel and A C Böhringer and C R von Bredow and F H Leinberger and M Kampschulte and L Maier and Y M von Bredow and V Flocke and H Merzendorfer and G A Krombach and A Vilcinskas and J Grimm and T E Trenczek and U Flögel}, doi = {10.1038/s41467-022-34865-7}, issn = {2041-1723}, year = {2022}, date = {2022-12-01}, journal = {Nat Commun}, volume = {13}, number = {1}, pages = {7216}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gao, Lu; Wang, Yanli; Abbas, Mureed; Zhang, Tingting; Ma, Enbo; Merzendorfer, Hans; Zhu, Kun Yan; Zhang, Jianzhen Insect Biochemistry and Molecular Biology, S. 103865, 2022, ISSN: 0965-1748. @article{GAO2022103865, title = {Both LmDicer-1 and two LmDicer-2s participate in siRNA-mediated RNAi pathway and contribute to high gene silencing efficiency in Locusta migratoria}, author = {Lu Gao and Yanli Wang and Mureed Abbas and Tingting Zhang and Enbo Ma and Hans Merzendorfer and Kun Yan Zhu and Jianzhen Zhang}, url = {https://www.sciencedirect.com/science/article/pii/S0965174822001473}, doi = {https://doi.org/10.1016/j.ibmb.2022.103865}, issn = {0965-1748}, year = {2022}, date = {2022-11-01}, journal = {Insect Biochemistry and Molecular Biology}, pages = {103865}, abstract = {Dicers belong to a class of large RNase III multidomain ribonucleases and are central components of the RNA interference (RNAi) pathways. In insects, Dicer-2 has been known to cleave long double-stranded RNA (dsRNA) in small interfering RNA (siRNA)-mediated-RNAi pathway. However, Dicer-1 is responsible for cleaving precursor microRNAs (pre28 miRNAs) in miRNA-mediated RNAi pathway. In this study, we identified one LmDicer-1 and two LmDicer-2 (LmDicer-2a and LmDicer-2b) genes in Locusta migratoria. The RNAi of RNAi assay showed that knockdown of each of the Dicer genes reduced RNAi efficiency against a target gene (Lmβ-Tubulin), suggesting that all these genes participated in the siRNA-mediated RNAi pathway. Sequence analyses of the siRNAs generated from dsLmβ-Tubulin after silencing each LmDicer gene showed no significant difference in the pattern of siRNAs mapped to dsLmβ-Tubulin. This result indicated that all the three LmDicers are capable of generating siRNAs from the dsRNA. We then generated recombinant proteins consisting of different domains using Escherichia coli expression system and incubated each recombinant protein with dsLmβ-Tubulin. We found that the recombinant Dicer proteins successfully cleaved dsLmβ-Tubulin. However, LmDicer-2a-R lacking dsRBD domain lost activity, suggesting that dsRBD domain is critical for Dicer function. Furthermore, overexpression of these proteins in Drosophila S2 cells improved RNAi efficiency. Our siRNA affinity chromatography and LC-MS/MS analysis identified LmDicer-2a, LmDicer-2b, LmR2D2, LmAgo2a, LmAgo1, LmStaufen and LmTARBP2 as constituents of RNA-induced silencing complex. Taken together, these data show that both LmDicer-1 and two LmDicer-2s all participate in siRNA-mediated RNAi pathway and likely contribute to high RNAi efficiency in L. migratoria.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dicers belong to a class of large RNase III multidomain ribonucleases and are central components of the RNA interference (RNAi) pathways. In insects, Dicer-2 has been known to cleave long double-stranded RNA (dsRNA) in small interfering RNA (siRNA)-mediated-RNAi pathway. However, Dicer-1 is responsible for cleaving precursor microRNAs (pre28 miRNAs) in miRNA-mediated RNAi pathway. In this study, we identified one LmDicer-1 and two LmDicer-2 (LmDicer-2a and LmDicer-2b) genes in Locusta migratoria. The RNAi of RNAi assay showed that knockdown of each of the Dicer genes reduced RNAi efficiency against a target gene (Lmβ-Tubulin), suggesting that all these genes participated in the siRNA-mediated RNAi pathway. Sequence analyses of the siRNAs generated from dsLmβ-Tubulin after silencing each LmDicer gene showed no significant difference in the pattern of siRNAs mapped to dsLmβ-Tubulin. This result indicated that all the three LmDicers are capable of generating siRNAs from the dsRNA. We then generated recombinant proteins consisting of different domains using Escherichia coli expression system and incubated each recombinant protein with dsLmβ-Tubulin. We found that the recombinant Dicer proteins successfully cleaved dsLmβ-Tubulin. However, LmDicer-2a-R lacking dsRBD domain lost activity, suggesting that dsRBD domain is critical for Dicer function. Furthermore, overexpression of these proteins in Drosophila S2 cells improved RNAi efficiency. Our siRNA affinity chromatography and LC-MS/MS analysis identified LmDicer-2a, LmDicer-2b, LmR2D2, LmAgo2a, LmAgo1, LmStaufen and LmTARBP2 as constituents of RNA-induced silencing complex. Taken together, these data show that both LmDicer-1 and two LmDicer-2s all participate in siRNA-mediated RNAi pathway and likely contribute to high RNAi efficiency in L. migratoria. |
Chen, Wei; Cao, Peng; Liu, Yuansheng; Yu, Ailing; Wang, Dong; Chen, Lei; Sundarraj, Rajamanikandan; Yuchi, Zhiguang; Gong, Yong; Merzendorfer, Hans; Yang, Qing Structural basis for directional chitin biosynthesis Artikel Nature, 2022, ISSN: 1476-4687. @article{RN14238, title = {Structural basis for directional chitin biosynthesis}, author = {Wei Chen and Peng Cao and Yuansheng Liu and Ailing Yu and Dong Wang and Lei Chen and Rajamanikandan Sundarraj and Zhiguang Yuchi and Yong Gong and Hans Merzendorfer and Qing Yang}, url = {https://doi.org/10.1038/s41586-022-05244-5}, doi = {10.1038/s41586-022-05244-5}, issn = {1476-4687}, year = {2022}, date = {2022-09-22}, journal = {Nature}, abstract = {Chitin, the most abundant aminopolysaccharide in nature, is an extracellular polymer consisting of N-acetylglucosamine (GlcNAc) units1. The key reactions of chitin biosynthesis are catalysed by chitin synthase2–4, a membrane-integrated glycosyltransferase that transfers GlcNAc from UDP-GlcNAc to a growing chitin chain. However, the precise mechanism of this process has yet to be elucidated. Here we report five cryo-electron microscopy structures of a chitin synthase from the devastating soybean root rot pathogenic oomycete Phytophthora sojae (PsChs1). They represent the apo, GlcNAc-bound, nascent chitin oligomer-bound, UDP-bound (post-synthesis) and chitin synthase inhibitor nikkomycin Z-bound states of the enzyme, providing detailed views into the multiple steps of chitin biosynthesis and its competitive inhibition. The structures reveal the chitin synthesis reaction chamber that has the substrate-binding site, the catalytic centre and the entrance to the polymer-translocating channel that allows the product polymer to be discharged. This arrangement reflects consecutive key events in chitin biosynthesis from UDP-GlcNAc binding and polymer elongation to the release of the product. We identified a swinging loop within the chitin-translocating channel, which acts as a ‘gate lock’ that prevents the substrate from leaving while directing the product polymer into the translocating channel for discharge to the extracellular side of the cell membrane. This work reveals the directional multistep mechanism of chitin biosynthesis and provides a structural basis for inhibition of chitin synthesis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chitin, the most abundant aminopolysaccharide in nature, is an extracellular polymer consisting of N-acetylglucosamine (GlcNAc) units1. The key reactions of chitin biosynthesis are catalysed by chitin synthase2–4, a membrane-integrated glycosyltransferase that transfers GlcNAc from UDP-GlcNAc to a growing chitin chain. However, the precise mechanism of this process has yet to be elucidated. Here we report five cryo-electron microscopy structures of a chitin synthase from the devastating soybean root rot pathogenic oomycete Phytophthora sojae (PsChs1). They represent the apo, GlcNAc-bound, nascent chitin oligomer-bound, UDP-bound (post-synthesis) and chitin synthase inhibitor nikkomycin Z-bound states of the enzyme, providing detailed views into the multiple steps of chitin biosynthesis and its competitive inhibition. The structures reveal the chitin synthesis reaction chamber that has the substrate-binding site, the catalytic centre and the entrance to the polymer-translocating channel that allows the product polymer to be discharged. This arrangement reflects consecutive key events in chitin biosynthesis from UDP-GlcNAc binding and polymer elongation to the release of the product. We identified a swinging loop within the chitin-translocating channel, which acts as a ‘gate lock’ that prevents the substrate from leaving while directing the product polymer into the translocating channel for discharge to the extracellular side of the cell membrane. This work reveals the directional multistep mechanism of chitin biosynthesis and provides a structural basis for inhibition of chitin synthesis. |
Chen, J; Zou, X; Zhu, W; Duan, Y; Merzendorfer, H; Zhao, Z; Yang, Q Insect Biochem Mol Biol, 149 , S. 103845, 2022, ISSN: 1879-0240 (Electronic) 0965-1748 (Linking). @article{RN1, title = {Fatty acid binding protein is required for chitin biosynthesis in the wing of Drosophila melanogaster}, author = {J Chen and X Zou and W Zhu and Y Duan and H Merzendorfer and Z Zhao and Q Yang}, url = {https://www.ncbi.nlm.nih.gov/pubmed/36165873}, doi = {10.1016/j.ibmb.2022.103845}, issn = {1879-0240 (Electronic) 0965-1748 (Linking)}, year = {2022}, date = {2022-09-01}, journal = {Insect Biochem Mol Biol}, volume = {149}, pages = {103845}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Vogt, Roland; Hartmann, Sarah; Kunze, Jan; Jupke, Jonathan Frederik; Steinhoff, Benedikt; Schönherr, Holger; Kuhnert, Klaus-Dieter; Witte, Klaudia; Lamatsch, Dunja Katharina; Wanzenböck, Josef Journal of Toxicology and Environmental Health, Part A, 0 , S. 1-14, 2022. @article{Vogt2022, title = {Silver nanoparticles adversely affect the swimming behavior of European Whitefish (Coregonus lavaretus) larvae within the low µg/L range}, author = {Roland Vogt and Sarah Hartmann and Jan Kunze and Jonathan Frederik Jupke and Benedikt Steinhoff and Holger Schönherr and Klaus-Dieter Kuhnert and Klaudia Witte and Dunja Katharina Lamatsch and Josef Wanzenböck}, editor = {Taylor Francis Online}, url = {https://www.tandfonline.com/doi/abs/10.1080/15287394.2022.2102099}, doi = {10.1080/15287394.2022.2102099}, year = {2022}, date = {2022-06-26}, journal = {Journal of Toxicology and Environmental Health, Part A}, volume = {0}, pages = {1-14}, abstract = {The aim of this study was to determine the effects of silver nanoparticles (AgNPs; speciation: NM-300 K) in the lab on the behavior of larvae in European Whitefish (Coregonus lavaretus), a relevant model species for temperate aquatic environments during alternating light and darkness phases. The behavioral parameters measured included activity, turning rate, and distance moved. C. lavaretus were exposed to AgNP at nominal concentrations of 0, 5, 15, 45, 135, or 405 µg/L (n = 33, each) and behavior was recorded using a custom-built tracking system equipped with light sources that reliably simulate light and darkness. The observed behavior was analyzed using generalized linear mixed models, which enabled reliable detection of AgNP-related movement patterns at 10-fold higher sensitivity compared to recently reported standard toxicological studies. Exposure to 45 µg/L AgNPs significantly resulted in hyperactive response patterns for both activity and turning rates after an illumination change from light to darkness suggesting that exposure to this compound triggered escape mechanisms and disorientation-like behaviors in C. lavaretus fish larvae. Even at 5 µg/L AgNPs some behavioral effects were detected, but further tests are required to assess their ecological relevance. Further, the behavior of fish larvae exposed to 135 µg/L AgNPs was comparable to the control for all test parameters, suggesting a triphasic dose response pattern. Data demonstrated the potential of combining generalized linear mixed models with behavioral investigations to detect adverse effects on aquatic species that might be overlooked using standard toxicological tests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The aim of this study was to determine the effects of silver nanoparticles (AgNPs; speciation: NM-300 K) in the lab on the behavior of larvae in European Whitefish (Coregonus lavaretus), a relevant model species for temperate aquatic environments during alternating light and darkness phases. The behavioral parameters measured included activity, turning rate, and distance moved. C. lavaretus were exposed to AgNP at nominal concentrations of 0, 5, 15, 45, 135, or 405 µg/L (n = 33, each) and behavior was recorded using a custom-built tracking system equipped with light sources that reliably simulate light and darkness. The observed behavior was analyzed using generalized linear mixed models, which enabled reliable detection of AgNP-related movement patterns at 10-fold higher sensitivity compared to recently reported standard toxicological studies. Exposure to 45 µg/L AgNPs significantly resulted in hyperactive response patterns for both activity and turning rates after an illumination change from light to darkness suggesting that exposure to this compound triggered escape mechanisms and disorientation-like behaviors in C. lavaretus fish larvae. Even at 5 µg/L AgNPs some behavioral effects were detected, but further tests are required to assess their ecological relevance. Further, the behavior of fish larvae exposed to 135 µg/L AgNPs was comparable to the control for all test parameters, suggesting a triphasic dose response pattern. Data demonstrated the potential of combining generalized linear mixed models with behavioral investigations to detect adverse effects on aquatic species that might be overlooked using standard toxicological tests. |
Muthukrishnan, Subbaratnam; Arakane, Yasuyuki; Noh, Mi Young; Mun, Seulgi; Merzendorfer, Hans; Boehringer, Christina; Wellmeyer, Benedikt; Yang, Qing; Qu, Mingbo; Liu, Lin Chitin in insect cuticle Artikel Adv. Insect Physiol., in press., 2022, ISSN: 0065-2806. @article{Liu2022, title = {Chitin in insect cuticle}, author = {Subbaratnam Muthukrishnan and Yasuyuki Arakane and Mi Young Noh and Seulgi Mun and Hans Merzendorfer and Christina Boehringer and Benedikt Wellmeyer and Qing Yang and Mingbo Qu and Lin Liu}, url = {https://doi.org/10.1016/bs.aiip.2022.03.001}, doi = {10.1016/bs.aiip.2022.03.001}, issn = {0065-2806}, year = {2022}, date = {2022-04-29}, journal = {Adv. Insect Physiol., in press.}, abstract = {Chitin, a linear structural polysaccharide is a major constituent of insect procuticle. Chitin is embedded in a complex matrix of proteins, which contributes significantly to the physicochemical and physiological properties of the cuticle. The wide range of properties of individual cuticles at specific anatomical locations and developmental stages is attributable to difference in chitin/protein ratio, protein composition of the matrix, cross-linking, presence of minerals and the degree of dehydration. The epidermal cells that underlie the cuticle orchestrate the timing of appearance of individual layers of the cuticle and its composition and organization. This process is particularly important during molting, when the old cuticle is replaced by a new one. Molting fluid contains an assortment of enzymes that deproteinize the cuticular matrix to expose the chitin crystallites to chitinolytic enzymes. A variety of enzymes of chitin metabolism participate in the synthesis, modification, and turnover of cuticular components and in cross-linking and tanning of proteins. Chemical inhibitors and RNA interference strategies targeted against the chitin metabolic enzymes and cuticular proteins represent specific and effective strategies for insect control.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Chitin, a linear structural polysaccharide is a major constituent of insect procuticle. Chitin is embedded in a complex matrix of proteins, which contributes significantly to the physicochemical and physiological properties of the cuticle. The wide range of properties of individual cuticles at specific anatomical locations and developmental stages is attributable to difference in chitin/protein ratio, protein composition of the matrix, cross-linking, presence of minerals and the degree of dehydration. The epidermal cells that underlie the cuticle orchestrate the timing of appearance of individual layers of the cuticle and its composition and organization. This process is particularly important during molting, when the old cuticle is replaced by a new one. Molting fluid contains an assortment of enzymes that deproteinize the cuticular matrix to expose the chitin crystallites to chitinolytic enzymes. A variety of enzymes of chitin metabolism participate in the synthesis, modification, and turnover of cuticular components and in cross-linking and tanning of proteins. Chemical inhibitors and RNA interference strategies targeted against the chitin metabolic enzymes and cuticular proteins represent specific and effective strategies for insect control. |
Wellbrock, Arndt; Eckhardt, Luca; Kelsey, Natalie; Heldmaier, Gerhard; Rozman, Jan; Witte, Klaudia Biol. Lett., 18: 20210675 , 2022. @article{AHJ2022, title = {Cool birds: first evidence of energy-saving nocturnal torpor in free-living common swifts Apus apus resting in their nests}, author = {Arndt Wellbrock and Luca Eckhardt and Natalie Kelsey and Gerhard Heldmaier and Jan Rozman and Klaudia Witte}, doi = {10.1098/rsbl.2021.0675}, year = {2022}, date = {2022-04-29}, journal = {Biol. Lett.}, volume = {18: 20210675}, abstract = {Daily torpor is a means of saving energy by controlled lowering of the metabolic rate (MR) during resting, usually coupled with a decrease in body temperature. We studied nocturnal daily torpor under natural conditions in free-living common swifts Apus apus resting in their nests as a family using two non-invasive approaches. First, we monitored nest temperature (Tnest) in up to 50 occupied nests per breeding season in 2010-2015. Drops in Tnest were the first indication of torpor. Among 16 673 observations, we detected 423 events of substantial drops in Tnest of on average 8.6°C. Second, we measured MR of the families inside nest-boxes prepared for calorimetric measurements during cold periods in the breeding seasons of 2017 and 2018. We measured oxygen consumption and carbon dioxide production using a mobile indirect respirometer and calculated the percentage reduction in MR. During six torpor events observed, MR was gradually reduced by on average 56% from the reference value followed by a decrease in Tnest of on average 7.6°C. By contrast, MR only decreased by about 33% on nights without torpor. Our field data gave an indication of daily torpor, which is used as a strategy for energy saving in free-living common swifts. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Daily torpor is a means of saving energy by controlled lowering of the metabolic rate (MR) during resting, usually coupled with a decrease in body temperature. We studied nocturnal daily torpor under natural conditions in free-living common swifts Apus apus resting in their nests as a family using two non-invasive approaches. First, we monitored nest temperature (Tnest) in up to 50 occupied nests per breeding season in 2010-2015. Drops in Tnest were the first indication of torpor. Among 16 673 observations, we detected 423 events of substantial drops in Tnest of on average 8.6°C. Second, we measured MR of the families inside nest-boxes prepared for calorimetric measurements during cold periods in the breeding seasons of 2017 and 2018. We measured oxygen consumption and carbon dioxide production using a mobile indirect respirometer and calculated the percentage reduction in MR. During six torpor events observed, MR was gradually reduced by on average 56% from the reference value followed by a decrease in Tnest of on average 7.6°C. By contrast, MR only decreased by about 33% on nights without torpor. Our field data gave an indication of daily torpor, which is used as a strategy for energy saving in free-living common swifts. |
Shi, XK; Li, S; and Lin, Y; Liu, XJ; Merzendorfer, H; Zhu, KY; Zhang, JZ Clathrin heavy chain is essential for the development and reproduction of Locusta migratoria Artikel Insect Science, in press., 2022. @article{Shi2022b, title = {Clathrin heavy chain is essential for the development and reproduction of Locusta migratoria}, author = {XK Shi and S Li and and Y Lin and XJ Liu and H Merzendorfer and KY Zhu and JZ Zhang}, year = {2022}, date = {2022-03-01}, journal = {Insect Science, in press.}, abstract = {Clathrin heavy chain (Chc) is a constituent of clathrin-coated vesicles and serves important functions in endocytosis and intracellular membrane trafficking but appears to have physiological roles also at the organismal level. Most of what we know about Chc functions originates from studies performed in fungal or vertebrate cells. However, the physiological functions of Chc in insects remain poorly understood. Here, we identified a Chc ortholog from a Locusta migratoria transcriptome database. RT-qPCR revealed that LmChc was constitutively expressed in fifth-instar nymphs. In this developmental stage, LmChc showed the highest expression in the ovary followed by hemolymph, testis, hindgut, midgut and foregut. In isolated hemocytes, we detected the Chc protein in patches at the plasma membrane. To examine the role of LmChc in L. migratoria during development, RNA interference was performed by injecting dsRNA into the early fifth-instar nymphs. Silencing of LmChc caused a lethal phenotype with molting defect from nymph to adult. In addition, silencing of LmChc resulted in abnormal development of the ovaries, the size of which was significantly smaller than that in controls. Taken together, our results suggest that LmChc is a vital gene in L. migratoria that plays an important role in growth, development and reproduction. LmChc may be used as an efficient RNAi target gene for developing dsRNA-based biological insecticides to manage insect pests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Clathrin heavy chain (Chc) is a constituent of clathrin-coated vesicles and serves important functions in endocytosis and intracellular membrane trafficking but appears to have physiological roles also at the organismal level. Most of what we know about Chc functions originates from studies performed in fungal or vertebrate cells. However, the physiological functions of Chc in insects remain poorly understood. Here, we identified a Chc ortholog from a Locusta migratoria transcriptome database. RT-qPCR revealed that LmChc was constitutively expressed in fifth-instar nymphs. In this developmental stage, LmChc showed the highest expression in the ovary followed by hemolymph, testis, hindgut, midgut and foregut. In isolated hemocytes, we detected the Chc protein in patches at the plasma membrane. To examine the role of LmChc in L. migratoria during development, RNA interference was performed by injecting dsRNA into the early fifth-instar nymphs. Silencing of LmChc caused a lethal phenotype with molting defect from nymph to adult. In addition, silencing of LmChc resulted in abnormal development of the ovaries, the size of which was significantly smaller than that in controls. Taken together, our results suggest that LmChc is a vital gene in L. migratoria that plays an important role in growth, development and reproduction. LmChc may be used as an efficient RNAi target gene for developing dsRNA-based biological insecticides to manage insect pests. |
Liu, XJ; Liang, XY; Guo, J; Shi, XK; Merzendorfer, H; Zhu, KY; Zhang, JZ V-ATPase subunit a is required for survival and midgut development of Locusta migratoria Artikel Insect Mol Biol. https://doi.10.1111/imb.12738, 31 , S. 60-72, 2022. @article{XJ2021, title = {V-ATPase subunit a is required for survival and midgut development of Locusta migratoria}, author = { XJ Liu and XY Liang and J Guo and XK Shi and H Merzendorfer and KY Zhu and JZ Zhang}, doi = { doi: 10.1111/imb.12738}, year = {2022}, date = {2022-02-01}, journal = {Insect Mol Biol. https://doi.10.1111/imb.12738}, volume = {31}, pages = {60-72}, abstract = {The vacuolar-type H+ -ATPase (V-ATPase) is an ATP-dependent proton pump, which regulates various cellular processes. To date, most functional studies on V-ATPases of insects have focused on subunits of the V1 complex, and there is little information on the VO genes. In this study, two cDNA sequences of LmV-ATPase a were identified in Locusta migratoria. RT-qPCR analysis revealed that LmV-ATPase a1 and LmV-ATPase a2 are differentially expressed in various tissues and developmental stages. Injection of dsRNA for the common region of LmV-ATPase a1 and LmV-ATPase a2 into third-instar nymphs resulted in a significant suppression of LmV-ATPase a. The injected nymphs ceased feeding, lost body weight and finally died at a mortality of 98.6%. Furthermore, aberrations of midgut epithelial cells, the accumulation of electron-lucent vesicles in the cytoplasm, and a partially damaged brush border were observed in dsLmV-ATPase a-injected nymphs using transmission electron microscopy. Especially, the mRNA level of wingles, and notch genes were dramatically down-regulated in the dsLmV-ATPase a-injected nymphs. Taken together, our results suggest that LmV-ATPase a is required for survival and midgut development of L. migratoria. Hence, this gene could be a good target for RNAi-based control against locusts. }, keywords = {}, pubstate = {published}, tppubtype = {article} } The vacuolar-type H+ -ATPase (V-ATPase) is an ATP-dependent proton pump, which regulates various cellular processes. To date, most functional studies on V-ATPases of insects have focused on subunits of the V1 complex, and there is little information on the VO genes. In this study, two cDNA sequences of LmV-ATPase a were identified in Locusta migratoria. RT-qPCR analysis revealed that LmV-ATPase a1 and LmV-ATPase a2 are differentially expressed in various tissues and developmental stages. Injection of dsRNA for the common region of LmV-ATPase a1 and LmV-ATPase a2 into third-instar nymphs resulted in a significant suppression of LmV-ATPase a. The injected nymphs ceased feeding, lost body weight and finally died at a mortality of 98.6%. Furthermore, aberrations of midgut epithelial cells, the accumulation of electron-lucent vesicles in the cytoplasm, and a partially damaged brush border were observed in dsLmV-ATPase a-injected nymphs using transmission electron microscopy. Especially, the mRNA level of wingles, and notch genes were dramatically down-regulated in the dsLmV-ATPase a-injected nymphs. Taken together, our results suggest that LmV-ATPase a is required for survival and midgut development of L. migratoria. Hence, this gene could be a good target for RNAi-based control against locusts. |
Kottaipalayam-Somasundaram, Sowmiya; Jacob, John; Aiyar, Balasubramaniam; Merzendorfer, Hans; Nambiar-Veetil, Mathish Pest Manag Sci, 78 , S. 296-303, 2022. @article{Kottaipalayam-Somasundaram2021, title = {Chitin metabolism as a potential target for RNAi-based control of the forestry pest \textit{Hyblaea puera} Cramer (Lepidoptera: Hyblaeidae) }, author = {Sowmiya Kottaipalayam-Somasundaram and John Jacob and Balasubramaniam Aiyar and Hans Merzendorfer and Mathish Nambiar-Veetil}, doi = {doi: 10.1002/ps.6634}, year = {2022}, date = {2022-01-01}, journal = {Pest Manag Sci}, volume = {78}, pages = {296-303}, abstract = {BACKGROUND Hyblaea puera, commonly known as the teak defoliator, is a serious pest in teak plantations. Despite the availability of control measures, this pest causes losses in yield and quality of timber through voracious feeding. RNA interference (RNAi) is a promising strategy for the control of this pest. Chitin metabolism, which is vital for the growth and development of arthropods, is a potential target for developing RNAi-based insecticides. RESULTS To assess the effects of chitin metabolism inhibition, H. puera larvae were treated with a chitin synthesis inhibitor, diflubenzuron (DFB). DFB treatment caused pupal deformities and disrupted eclosion. Partial gene sequences for three key genes of H. puera chitin metabolism were cloned and sequenced: chitin synthase 1 (HpCHS1), chitinase-h (HpChi-h) and ecdysone receptor (HpEcR). Feeding dsRNA cognate for these three target genes to the first instar of H. puera resulted in mortality and reduction in the corresponding transcript levels as assessed through qRT-PCR. This is the first report of RNAi in this forestry pest. The highest mortality was 45.9%, in response to dsHpEcR treatment; HpChi-h transcripts were the most down-regulated in response to dsHpEcR feeding. DsHpEcR RNAi resulted in growth inhibition and molting arrest. The mortalities were 29.7% and 32.4% for dsHpCHS1 and dsHpChi-h feeding, respectively. CONCLUSION Chitin metabolism could be a potential target for RNAi-based control of H. puera, and HpCHS1, HpChi-h and HpEcR could be suitable target genes. However, the RNAi efficacy needs to be improved through formulations that improve stability and uptake, and employing better delivery strategies. }, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND Hyblaea puera, commonly known as the teak defoliator, is a serious pest in teak plantations. Despite the availability of control measures, this pest causes losses in yield and quality of timber through voracious feeding. RNA interference (RNAi) is a promising strategy for the control of this pest. Chitin metabolism, which is vital for the growth and development of arthropods, is a potential target for developing RNAi-based insecticides. RESULTS To assess the effects of chitin metabolism inhibition, H. puera larvae were treated with a chitin synthesis inhibitor, diflubenzuron (DFB). DFB treatment caused pupal deformities and disrupted eclosion. Partial gene sequences for three key genes of H. puera chitin metabolism were cloned and sequenced: chitin synthase 1 (HpCHS1), chitinase-h (HpChi-h) and ecdysone receptor (HpEcR). Feeding dsRNA cognate for these three target genes to the first instar of H. puera resulted in mortality and reduction in the corresponding transcript levels as assessed through qRT-PCR. This is the first report of RNAi in this forestry pest. The highest mortality was 45.9%, in response to dsHpEcR treatment; HpChi-h transcripts were the most down-regulated in response to dsHpEcR feeding. DsHpEcR RNAi resulted in growth inhibition and molting arrest. The mortalities were 29.7% and 32.4% for dsHpCHS1 and dsHpChi-h feeding, respectively. CONCLUSION Chitin metabolism could be a potential target for RNAi-based control of H. puera, and HpCHS1, HpChi-h and HpEcR could be suitable target genes. However, the RNAi efficacy needs to be improved through formulations that improve stability and uptake, and employing better delivery strategies. |
Duan, Yanwei; Zhu, Weixing; Zhao, Xiaoming; Merzendorfer, Hans; Chen, Jiqiang; Xu Zou and, Qing Yang Insect Biochem Mol Biol, 141 , S. 103718, 2022. @article{Duan2022, title = {Choline transporter-like protein 2 interacts with chitin synthase 1 and is involved in insect cuticle development}, author = {Yanwei Duan and Weixing Zhu and Xiaoming Zhao and Hans Merzendorfer and Jiqiang Chen and Xu Zou and, Qing Yang}, doi = { doi: 10.1016/j.ibmb.2021.103718.}, year = {2022}, date = {2022-01-01}, journal = {Insect Biochem Mol Biol}, volume = {141}, pages = {103718}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Shi, Xuekai; Liu, Xiaojian; Cooper, Anastasia M W; Silver, Kristopher; Merzendorfer, Hans; Zhu, Kun Yan; Zhang, Jianzhen Vacuolar (H+)-ATPase subunit c is essential for the survival and systemic RNA interference response in Locusta migratoria Artikel Pest Manag Sci, in press, 2022. @article{Shi2022, title = {Vacuolar (H+)-ATPase subunit c is essential for the survival and systemic RNA interference response in Locusta migratoria}, author = {Xuekai Shi and Xiaojian Liu and Anastasia M.W. Cooper and Kristopher Silver and Hans Merzendorfer and Kun Yan Zhu and Jianzhen Zhang }, year = {2022}, date = {2022-01-01}, journal = {Pest Manag Sci, in press}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Zhu, Weixing; Duan, Yanwei; Chen, Jiqiang; Merzendorfer, Hans; Zou, Xu; Yang, Qing SERCA interacts with chitin synthase and participates in cuticular chitin biogenesis in Drosophila Artikel Insect Biochemistry and Molecular Biology, 145 , S. 103783, 2022, ISSN: 0965-1748. @article{ZHU2022103783, title = {SERCA interacts with chitin synthase and participates in cuticular chitin biogenesis in Drosophila}, author = {Weixing Zhu and Yanwei Duan and Jiqiang Chen and Hans Merzendorfer and Xu Zou and Qing Yang}, url = {https://www.sciencedirect.com/science/article/pii/S0965174822000650}, doi = {https://doi.org/10.1016/j.ibmb.2022.103783}, issn = {0965-1748}, year = {2022}, date = {2022-01-01}, journal = {Insect Biochemistry and Molecular Biology}, volume = {145}, pages = {103783}, abstract = {The biogenesis of chitin, a major structural polysaccharide found in the cuticle and peritrophic matrix, is crucial for insect growth and development. Chitin synthase, a membrane-integral β-glycosyltransferase, has been identified as the core of the chitin biogenesis machinery. However, a yet unknown number of auxiliary proteins appear to assist in chitin biosynthesis, whose precise function remains elusive. Here, we identified a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), in the fruit fly Drosophila melanogaster, as a chitin biogenesis-associated protein. The physical interaction between DmSERCA and epidermal chitin synthase (Krotzkopf verkehrt, Kkv) was demonstrated and analyzed using split-ubiquitin membrane yeast two-hybrid, bimolecular fluorescent complementation, pull-down, and immunoprecipitation assays. The interaction involves N-terminal regions (aa 48–81 and aa 247–33) and C-terminal regions (aa 743–783 and aa 824–859) of DmSERCA and two N-terminal regions (aa 121–179 and aa 369–539) of Kkv, all of which are predicted be transmembrane helices. While tissue-specific knock-down of DmSERCA in the epidermis caused larval and pupal lethality, the knock-down of DmSERCA in wings resulted in smaller and crinkled wings, a significant decrease in chitin deposition, and the loss of chitin lamellar structure. Although DmSERCA is well-known for its role in muscular contraction, this study reveals a novel role in chitin synthesis, contributing to our knowledge on the machinery of chitin biogenesis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The biogenesis of chitin, a major structural polysaccharide found in the cuticle and peritrophic matrix, is crucial for insect growth and development. Chitin synthase, a membrane-integral β-glycosyltransferase, has been identified as the core of the chitin biogenesis machinery. However, a yet unknown number of auxiliary proteins appear to assist in chitin biosynthesis, whose precise function remains elusive. Here, we identified a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), in the fruit fly Drosophila melanogaster, as a chitin biogenesis-associated protein. The physical interaction between DmSERCA and epidermal chitin synthase (Krotzkopf verkehrt, Kkv) was demonstrated and analyzed using split-ubiquitin membrane yeast two-hybrid, bimolecular fluorescent complementation, pull-down, and immunoprecipitation assays. The interaction involves N-terminal regions (aa 48–81 and aa 247–33) and C-terminal regions (aa 743–783 and aa 824–859) of DmSERCA and two N-terminal regions (aa 121–179 and aa 369–539) of Kkv, all of which are predicted be transmembrane helices. While tissue-specific knock-down of DmSERCA in the epidermis caused larval and pupal lethality, the knock-down of DmSERCA in wings resulted in smaller and crinkled wings, a significant decrease in chitin deposition, and the loss of chitin lamellar structure. Although DmSERCA is well-known for its role in muscular contraction, this study reveals a novel role in chitin synthesis, contributing to our knowledge on the machinery of chitin biogenesis. |
Rösner, J; Merzendorfer, H Insect Sci, 29 (4), S. 1096-1104, 2022, ISSN: 1744-7917 (Electronic) 1672-9609 (Linking). @article{RN7b, title = {Identification of two ABCC transporters involved in malathion detoxification in the red flour beetle, Tribolium castaneum}, author = {J Rösner and H Merzendorfer}, url = {https://www.ncbi.nlm.nih.gov/pubmed/34730283}, doi = {10.1111/1744-7917.12981}, issn = {1744-7917 (Electronic) 1672-9609 (Linking)}, year = {2022}, date = {2022-01-01}, journal = {Insect Sci}, volume = {29}, number = {4}, pages = {1096-1104}, abstract = {ABC transporters have been suggested to be involved in insecticide detoxification in different insect species mainly based on the indirect observation of transcriptional upregulation of ABC gene expression in response to insecticide exposure. Previous studies performed by us and others in the red flour beetle, Tribolium castaneum, have analyzed the function of TcABCA-C and TcABCG-H genes using RNA interference (RNAi) and demonstrated that specific TcABCA and TcABCC genes are involved in the elimination of the pyrethroid tefluthrin and the benzoylurea diflubenzuron, because gene silencing increased the beetle's susceptibility to the insecticides. In this study, we focused on the potential functions of TcABCA-C genes in detoxification of the pyrethroid cyfluthrin (CF), the organophosphate malathion (MAL) and the diacylhdyazine tebufenozide (TBF). Analysis of transcript levels of selected TcABCA-C genes in response to treatment with these three chemically unrelated insecticides revealed that some genes were particularly upregulated after insecticide treatment. In addition, the ABC inhibitor verapamil synergized significantly the toxicity of MAL but only negligibly CF and TBF toxicities. Finally, silencing of two TcABCC genes by RNAi revealed a significant increase in susceptibility to MAL. In contrast, we did not observe a significant increase in insecticide-induced mortalities when knocking down TcABC genes in larvae treated with CF or TBF, although they were upregulated in response to insecticide treatment. Our results suggest that two pleiotropic ABCC transporters expressed in metabolic and excretory tissues contribute to the elimination of MAL.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABC transporters have been suggested to be involved in insecticide detoxification in different insect species mainly based on the indirect observation of transcriptional upregulation of ABC gene expression in response to insecticide exposure. Previous studies performed by us and others in the red flour beetle, Tribolium castaneum, have analyzed the function of TcABCA-C and TcABCG-H genes using RNA interference (RNAi) and demonstrated that specific TcABCA and TcABCC genes are involved in the elimination of the pyrethroid tefluthrin and the benzoylurea diflubenzuron, because gene silencing increased the beetle's susceptibility to the insecticides. In this study, we focused on the potential functions of TcABCA-C genes in detoxification of the pyrethroid cyfluthrin (CF), the organophosphate malathion (MAL) and the diacylhdyazine tebufenozide (TBF). Analysis of transcript levels of selected TcABCA-C genes in response to treatment with these three chemically unrelated insecticides revealed that some genes were particularly upregulated after insecticide treatment. In addition, the ABC inhibitor verapamil synergized significantly the toxicity of MAL but only negligibly CF and TBF toxicities. Finally, silencing of two TcABCC genes by RNAi revealed a significant increase in susceptibility to MAL. In contrast, we did not observe a significant increase in insecticide-induced mortalities when knocking down TcABC genes in larvae treated with CF or TBF, although they were upregulated in response to insecticide treatment. Our results suggest that two pleiotropic ABCC transporters expressed in metabolic and excretory tissues contribute to the elimination of MAL. |
Wellbrock, Arndt H J; Witte, Klaudia Movement Ecology, 10 (1), S. 58, 2022, ISSN: 2051-3933. @article{RN14340, title = {No “carry-over” effects of tracking devices on return rate and parameters determining reproductive success in once and repeatedly tagged common swifts (Apus apus), a long-distance migratory bird}, author = {Arndt H J Wellbrock and Klaudia Witte}, url = {https://doi.org/10.1186/s40462-022-00357-y}, doi = {10.1186/s40462-022-00357-y}, issn = {2051-3933}, year = {2022}, date = {2022-01-01}, journal = {Movement Ecology}, volume = {10}, number = {1}, pages = {58}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Nabariya, D K; Heinz, A; Derksen, S; Krauß, S Front Mol Biosci, 9 , S. 1000932, 2022, ISSN: 2296-889X (Print) 2296-889x. @article{RN13762, title = {Intracellular and intercellular transport of RNA organelles in CXG repeat disorders: The strength of weak ties}, author = {D K Nabariya and A Heinz and S Derksen and S Krauß}, doi = {10.3389/fmolb.2022.1000932}, issn = {2296-889X (Print) 2296-889x}, year = {2022}, date = {2022-01-01}, journal = {Front Mol Biosci}, volume = {9}, pages = {1000932}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Vogt, R; Hartmann, S; Kunze, J; Jupke, J F; Steinhoff, B; Schönherr, H; Kuhnert, K D; Witte, K; Lamatsch, D K; Wanzenböck, J J Toxicol Environ Health A, 85 (21), S. 867-880, 2022, ISSN: 1528-7394 (Print) 0098-4108. @article{RN14413, title = {Silver nanoparticles adversely affect the swimming behavior of European Whitefish (Coregonus lavaretus) larvae within the low µg/L range}, author = {R Vogt and S Hartmann and J Kunze and J F Jupke and B Steinhoff and H Schönherr and K D Kuhnert and K Witte and D K Lamatsch and J Wanzenböck}, doi = {10.1080/15287394.2022.2102099}, issn = {1528-7394 (Print) 0098-4108}, year = {2022}, date = {2022-01-01}, journal = {J Toxicol Environ Health A}, volume = {85}, number = {21}, pages = {867-880}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2021 |
van Heinz Annika Schilling Judith, Roon-Mom Willeke Krauss Sybille The MID1 Protein: A Promising Therapeutic Target in Huntington’s Disease Artikel Forntiers in Genetics, 2021. @article{Krauss2021b, title = {The MID1 Protein: A Promising Therapeutic Target in Huntington’s Disease}, author = {Heinz Annika, Schilling Judith, van Roon-Mom Willeke, Krauss Sybille}, doi = {https://doi.org/10.3389/fgene.2021.761714}, year = {2021}, date = {2021-10-01}, journal = {Forntiers in Genetics}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A, Heinz; DK, Nabariya; S., Krauss Huntingtin and Its Role in Mechanisms of RNA-Mediated Toxicity Artikel Toxins (Basel), 2021. @article{Krauss2021b, title = {Huntingtin and Its Role in Mechanisms of RNA-Mediated Toxicity}, author = {Heinz A and Nabariya DK and Krauss S.}, doi = {https://doi.org/10.3390/toxins13070487}, year = {2021}, date = {2021-07-14}, journal = {Toxins (Basel)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A., Heinz; D.K., Nabariya; S, Krauß Huntington’s Disease and Neurodegeneration Artikel Kostrzewa R.M. (eds) Handbook of Neurotoxicity. Springer, Cham. , 2021. @article{Krauss2021b, title = {Huntington’s Disease and Neurodegeneration}, author = {Heinz A. and Nabariya D.K. and Krauß S}, doi = {https://doi.org/10.1007/978-3-030-71519-9_226-1}, year = {2021}, date = {2021-06-25}, journal = {Kostrzewa R.M. (eds) Handbook of Neurotoxicity. Springer, Cham. }, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Qu, Mingbo; Merzendorfer, Hans; Moussian, Bernard; Yang, Qing Bioinsecticides as future mainstream pest control agents: Opportunities and challenges Artikel Front. Agr. Sci. Eng., 404 (0), S. 1-17, 2021, ISBN: 2095-7505, (na). @article{Qu2021, title = {Bioinsecticides as future mainstream pest control agents: Opportunities and challenges}, author = {Mingbo Qu and Hans Merzendorfer and Bernard Moussian and Qing Yang}, editor = {Front J}, url = { https://doi.org/10.15302/J-FASE-2021404 }, doi = {10.15302/J-FASE-2021404 }, isbn = {2095-7505}, year = {2021}, date = {2021-06-04}, journal = {Front. Agr. Sci. Eng.}, volume = {404}, number = {0}, pages = {1-17}, abstract = {Bioinsecticides are naturally occurring substances from different sources that control insect pests. Ideal bioinsecticides should exhibit low toxicity to non-target organisms. Further, they should be easily degraded in sewage plants and natural environments, highly effective in small quantities and only affect target pests. Public concerns about possible side-effects of conventional pesticides has accelerated bioinsecticide research and development. However, to develop bioinsecticides into a mainstream product line, the high production costs, short shelflife and uncertain acting modes of many bioinsecticides need to be considered. In this review, we summarize current progress on bioinsecticides, which are categorized into three classes, i.e. biochemical insecticides and their derivatives, plant-incorparated protectants (PIPs), and microbial bioinsecticides. The shortcomings that prevent bioinsecticides from being widely applied are discussed, and the future research direction in the field is proposed.}, note = {na}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bioinsecticides are naturally occurring substances from different sources that control insect pests. Ideal bioinsecticides should exhibit low toxicity to non-target organisms. Further, they should be easily degraded in sewage plants and natural environments, highly effective in small quantities and only affect target pests. Public concerns about possible side-effects of conventional pesticides has accelerated bioinsecticide research and development. However, to develop bioinsecticides into a mainstream product line, the high production costs, short shelflife and uncertain acting modes of many bioinsecticides need to be considered. In this review, we summarize current progress on bioinsecticides, which are categorized into three classes, i.e. biochemical insecticides and their derivatives, plant-incorparated protectants (PIPs), and microbial bioinsecticides. The shortcomings that prevent bioinsecticides from being widely applied are discussed, and the future research direction in the field is proposed. |
DV, Berdnikova; P, Carloni; S, Krauß; G., Rossetti Molecules, 2021. @article{Krauss2021b, title = {Role and Perspective of Molecular Simulation-Based Investigation of RNA-Ligand Interaction: From Small Molecules and Peptides to Photoswitchable RNA Binding}, author = {Berdnikova DV and Carloni P and Krauß S and Rossetti G.}, doi = {https://doi.org/10.3390/molecules26113384}, year = {2021}, date = {2021-06-03}, journal = {Molecules}, abstract = {Abstract Aberrant RNA–protein complexes are formed in a variety of diseases. Identifying the ligands that interfere with their formation is a valuable therapeutic strategy. Molecular simulation, validated against experimental data, has recently emerged as a powerful tool to predict both the pose and energetics of such ligands. Thus, the use of molecular simulation may provide insight into aberrant molecular interactions in diseases and, from a drug design perspective, may allow for the employment of less wet lab resources than traditional in vitro compound screening approaches. With regard to basic research questions, molecular simulation can support the understanding of the exact molecular interaction and binding mode. Here, we focus on examples targeting RNA–protein complexes in neurodegenerative diseases and viral infections. These examples illustrate that the strategy is rather general and could be applied to different pharmacologically relevant approaches. We close this study by outlining one of these approaches, namely the light-controllable association of small molecules with RNA, as an emerging approach in RNA-targeting therapy. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract Aberrant RNA–protein complexes are formed in a variety of diseases. Identifying the ligands that interfere with their formation is a valuable therapeutic strategy. Molecular simulation, validated against experimental data, has recently emerged as a powerful tool to predict both the pose and energetics of such ligands. Thus, the use of molecular simulation may provide insight into aberrant molecular interactions in diseases and, from a drug design perspective, may allow for the employment of less wet lab resources than traditional in vitro compound screening approaches. With regard to basic research questions, molecular simulation can support the understanding of the exact molecular interaction and binding mode. Here, we focus on examples targeting RNA–protein complexes in neurodegenerative diseases and viral infections. These examples illustrate that the strategy is rather general and could be applied to different pharmacologically relevant approaches. We close this study by outlining one of these approaches, namely the light-controllable association of small molecules with RNA, as an emerging approach in RNA-targeting therapy. |
Furtado, Gabriel Vasata; Yang, Jing; Wu, Di; Papagiannopoulos, Christos I; Terpstra, Hanna M; Kuiper, Elsiena E F; Krauss, Sybille; Zhu, Wei-Guo; Kampinga, Harm H; Bergink, Steven Human Molecular Genetics, 2021, ( ). @article{Krauss2021, title = {FOXO1 controls protein synthesis and transcript abundance of mutant polyglutamine proteins, preventing protein aggregation.}, author = {Gabriel Vasata Furtado and Jing Yang and Di Wu and Christos I Papagiannopoulos and Hanna M Terpstra and E F Elsiena Kuiper and Sybille Krauss and Wei-Guo Zhu and Harm H Kampinga and Steven Bergink}, editor = { }, doi = {10.1093}, year = {2021}, date = {2021-06-01}, journal = {Human Molecular Genetics}, abstract = { Abstract FOXO1, a transcription factor downstream of the insulin/insulin like growth factor axis, has been linked to protein degradation. Elevated expression of FOXO orthologs can also prevent the aggregation of cytosine adenine guanine (CAG)-repeat disease causing polyglutamine (polyQ) proteins but whether FOXO1 targets mutant proteins for degradation is unclear. Here, we show that increased expression of FOXO1 prevents toxic polyQ aggregation in human cells while reducing FOXO1 levels has the opposite effect and accelerates it. Although FOXO1 indeed stimulates autophagy, its effect on polyQ aggregation is independent of autophagy, ubiquitin–proteasome system (UPS) mediated protein degradation and is not due to a change in mutant polyQ protein turnover. Instead, FOXO1 specifically downregulates protein synthesis rates from expanded pathogenic CAG repeat transcripts. FOXO1 orchestrates a change in the composition of proteins that occupy mutant expanded CAG transcripts, including the recruitment of IGF2BP3. This mRNA binding protein enables a FOXO1 driven decrease in pathogenic expanded CAG transcript- and protein levels, thereby reducing the initiation of amyloidogenesis. Our data thus demonstrate that FOXO1 not only preserves protein homeostasis at multiple levels, but also reduces the accumulation of aberrant RNA species that may co-contribute to the toxicity in CAG-repeat diseases.}, note = { }, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract FOXO1, a transcription factor downstream of the insulin/insulin like growth factor axis, has been linked to protein degradation. Elevated expression of FOXO orthologs can also prevent the aggregation of cytosine adenine guanine (CAG)-repeat disease causing polyglutamine (polyQ) proteins but whether FOXO1 targets mutant proteins for degradation is unclear. Here, we show that increased expression of FOXO1 prevents toxic polyQ aggregation in human cells while reducing FOXO1 levels has the opposite effect and accelerates it. Although FOXO1 indeed stimulates autophagy, its effect on polyQ aggregation is independent of autophagy, ubiquitin–proteasome system (UPS) mediated protein degradation and is not due to a change in mutant polyQ protein turnover. Instead, FOXO1 specifically downregulates protein synthesis rates from expanded pathogenic CAG repeat transcripts. FOXO1 orchestrates a change in the composition of proteins that occupy mutant expanded CAG transcripts, including the recruitment of IGF2BP3. This mRNA binding protein enables a FOXO1 driven decrease in pathogenic expanded CAG transcript- and protein levels, thereby reducing the initiation of amyloidogenesis. Our data thus demonstrate that FOXO1 not only preserves protein homeostasis at multiple levels, but also reduces the accumulation of aberrant RNA species that may co-contribute to the toxicity in CAG-repeat diseases. |
Rösner, Janin; Tietmeyer, Johanne; Merzendorfer, Hans Functional analysis of ABCG and ABCH transporters from the red flour beetle, Tribolium castaneum Artikel Pest Management Science, 77 (6), S. 2955-2963, 2021. @article{https://doi.org/10.1002/ps.6332, title = {Functional analysis of ABCG and ABCH transporters from the red flour beetle, Tribolium castaneum}, author = {Janin Rösner and Johanne Tietmeyer and Hans Merzendorfer}, url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.6332}, doi = {https://doi.org/10.1002/ps.6332}, year = {2021}, date = {2021-01-01}, journal = {Pest Management Science}, volume = {77}, number = {6}, pages = {2955-2963}, abstract = {Abstract BACKGROUND ATP-binding cassette transporter (ABC transporter) subfamilies ABCA–C and ABCG–H have been implicated in insecticide detoxification, mostly based on findings of elevated gene expression in response to insecticide treatment. We previously characterized TcABCA–C genes from the model beetle and pest Tribolium castaneum and demonstrated that TcABCA and TcABCC genes are involved in the elimination of diflubenzuron, because RNA interference (RNAi)-mediated gene silencing increased susceptibility. In this study, we focused on the potential functions of TcABCG and TcABCH genes in insecticide detoxification. RESULTS When we silenced the expression of TcABCG–H genes using RNAi, we noticed a previously unreported developmental RNAi phenotype for TcABCG-4F, which is characterized by 50% mortality and ecdysial arrest during adult moult. When we knocked down the Drosophila brown orthologue TcABCG-XC, we did not obtain apparent eye colour phenotypes but did observe a loss of riboflavin uptake by Malpighian tubules. Next, we determined the expression profiles of all TcABCG–H genes in different tissues and developmental stages and analysed transcript levels in response to treatment with four chemically unrelated insecticides. We found that some genes were specifically upregulated after insecticide treatment. However, when we determined insecticide-induced mortalities in larvae that were treated by double-stranded RNA injection to silence those TcABCG–H genes that were upregulated, we did not observe a significant increase in susceptibility to insecticides. CONCLUSION Our findings suggest that the observed insecticide-dependent induction of TcABCG–H gene expression reflects an unspecific stress response, and hence underlines the significance of functional studies on insecticide detoxification. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Abstract BACKGROUND ATP-binding cassette transporter (ABC transporter) subfamilies ABCA–C and ABCG–H have been implicated in insecticide detoxification, mostly based on findings of elevated gene expression in response to insecticide treatment. We previously characterized TcABCA–C genes from the model beetle and pest Tribolium castaneum and demonstrated that TcABCA and TcABCC genes are involved in the elimination of diflubenzuron, because RNA interference (RNAi)-mediated gene silencing increased susceptibility. In this study, we focused on the potential functions of TcABCG and TcABCH genes in insecticide detoxification. RESULTS When we silenced the expression of TcABCG–H genes using RNAi, we noticed a previously unreported developmental RNAi phenotype for TcABCG-4F, which is characterized by 50% mortality and ecdysial arrest during adult moult. When we knocked down the Drosophila brown orthologue TcABCG-XC, we did not obtain apparent eye colour phenotypes but did observe a loss of riboflavin uptake by Malpighian tubules. Next, we determined the expression profiles of all TcABCG–H genes in different tissues and developmental stages and analysed transcript levels in response to treatment with four chemically unrelated insecticides. We found that some genes were specifically upregulated after insecticide treatment. However, when we determined insecticide-induced mortalities in larvae that were treated by double-stranded RNA injection to silence those TcABCG–H genes that were upregulated, we did not observe a significant increase in susceptibility to insecticides. CONCLUSION Our findings suggest that the observed insecticide-dependent induction of TcABCG–H gene expression reflects an unspecific stress response, and hence underlines the significance of functional studies on insecticide detoxification. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. |
Rösner, Janin; Tietmeyer, Johanne; Merzendorfer, Hans Journal of Pest Science, 2021, ISSN: 1612-4766. @article{RN13788, title = {Organic anion-transporting polypeptides are involved in the elimination of insecticides from the red flour beetle, Tribolium castaneum}, author = {Janin Rösner and Johanne Tietmeyer and Hans Merzendorfer}, url = {https://doi.org/10.1007/s10340-020-01317-4}, doi = {10.1007/s10340-020-01317-4}, issn = {1612-4766}, year = {2021}, date = {2021-01-01}, journal = {Journal of Pest Science}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2020 |
Collison, A M; Sokulsky, L A; Nightingale, S; Percival, E; LeFevre, A; Meredith, J; Krauss, S; Mattes, Fosterand P S J In vivo targeting of miR‐223 in experimental eosinophilic oesophagitis Artikel 2020. @article{Mattes2020, title = {In vivo targeting of miR‐223 in experimental eosinophilic oesophagitis}, author = {A M Collison and L A Sokulsky and S Nightingale and E Percival and A LeFevre and J Meredith and S Krauss and P S Fosterand J Mattes}, editor = {Clinical & Translational Immunology }, url = {https://doi.org/10.1002/cti2.1210}, doi = {10.1002/cti2.1210}, year = {2020}, date = {2020-11-23}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fuss, T; Flöck, S; Witte, K Anim. Behav, 2020. @article{Witte2020, title = {Sex-specific cognitive flexibility in Atlantic mollies when learning from male demonstrators exploring a new food source.}, author = {T Fuss and S Flöck and K Witte }, doi = {https://doi.org/10.1016/j.anbehav.2020.12.012}, year = {2020}, date = {2020-11-02}, journal = {Anim. Behav}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Lountzi, D; Henzel, K; Jazbec, K; Banow, D; Krauss, S; Rozman, P; Ehinger, D 2020. @article{Lountzi2020, title = {Effects of heterochronic, non-myeloablative bone marrow transplantation on age-releated behavioural changes in mice}, author = { D Lountzi and K Henzel and K Jazbec and D Banow and S Krauss and P Rozman and D Ehinger }, editor = {Mechanisms of Ageing and Develpoment }, url = {https://doi.org/10.1016/j.mad.2020.111327}, doi = {10.1016/j.mad.2020.111327}, year = {2020}, date = {2020-10-01}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Hartmann, S; Beasly, A; Mozhayeva, D; Engelhard, C; Witte, K Scientific Reports, 2020. @article{Hartmann2020, title = {Defective defence in Daphnia daughters: silver nanoparticles inhibit anti-predator defence in offspring but not in maternal Daphnia magna}, author = {S Hartmann and A Beasly and D Mozhayeva and C Engelhard and K Witte}, doi = {https://doi.org/10.1038/s41598-020-64652-7}, year = {2020}, date = {2020-05-15}, journal = {Scientific Reports}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
I. M. Kureck, Witte K Habitatwahl von Mauerasseln und Kellerasseln - Wissenschaftliche Arbeitsweisen an einem konkreten Beispiel vermitteln. BU praktisch (in press) Artikel 2020. @article{Kureck2020b, title = {Habitatwahl von Mauerasseln und Kellerasseln - Wissenschaftliche Arbeitsweisen an einem konkreten Beispiel vermitteln. BU praktisch (in press)}, author = {I. M. Kureck, K. Witte}, year = {2020}, date = {2020-04-01}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Schaub, Tonio; Wellbrock, Arndt HJ; Rozman, Jan; Witte, Klaudia Bird Study, 66 (4), S. 519–530, 2020. @article{schaub2019light, title = {Light data from geolocation reveal patterns of nest visit frequency and suitable conditions for efficient nest site monitoring in Common Swifts Apus apus}, author = {Tonio Schaub and Arndt HJ Wellbrock and Jan Rozman and Klaudia Witte}, doi = {https://doi.org/10.1080/00063657.2020.1732862}, year = {2020}, date = {2020-03-10}, journal = {Bird Study}, volume = {66}, number = {4}, pages = {519--530}, publisher = {Taylor & Francis}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Kureck, Ilka; Witte, Klaudia Habitatwahl von Mauerasseln und Kellerasseln Artikel BU praktisch-Das Online-Journal für den Biologieunterricht, 3 (1), S. 4–4, 2020. @article{kureck2020habitatwahl, title = {Habitatwahl von Mauerasseln und Kellerasseln}, author = {Ilka Kureck and Klaudia Witte}, url = {https://www.bu-praktisch.de/index.php/bupraktisch/article/view/1749 https://video.uni-siegen.de/media_objects/1n79h429p https://video.uni-siegen.de/media_objects/5d86p025z}, year = {2020}, date = {2020-01-01}, journal = {BU praktisch-Das Online-Journal für den Biologieunterricht}, volume = {3}, number = {1}, pages = {4--4}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rosner, J; Wellmeyer, B; Merzendorfer, H Curr Pharm Des, 2020, ISSN: 1873-4286 (Electronic) 1381-6128 (Linking). @article{RN1_30, title = {Tribolium castaneum: a model for investigating the mode of action of insecticides and mechanisms of resistance}, author = {J Rosner and B Wellmeyer and H Merzendorfer}, url = {https://www.ncbi.nlm.nih.gov/pubmed/32400327}, doi = {10.2174/1381612826666200513113140}, issn = {1873-4286 (Electronic) 1381-6128 (Linking)}, year = {2020}, date = {2020-01-01}, journal = {Curr Pharm Des}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rosner, J; Merzendorfer, H Insect Biochem Mol Biol, 116 , S. 103282, 2020, ISSN: 1879-0240 (Electronic) 0965-1748 (Linking). @article{RN3g, title = {Transcriptional plasticity of different ABC transporter genes from Tribolium castaneum contributes to diflubenzuron resistance}, author = {J Rosner and H Merzendorfer}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31740345}, doi = {10.1016/j.ibmb.2019.103282}, issn = {1879-0240 (Electronic) 0965-1748 (Linking)}, year = {2020}, date = {2020-01-01}, journal = {Insect Biochem Mol Biol}, volume = {116}, pages = {103282}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Brlik, V; Kolecek, J; Burgess, M; Hahn, S; Humple, D; Krist, M; Ouwehand, J; Weiser, E L; Adamik, P; Alves, J A; Arlt, D; Barisic, S; Becker, D; Belda, E J; Beran, V; Both, C; Bravo, S P; Briedis, M; Chutny, B; Cikovic, D; Cooper, N W; Costa, J S; Cueto, V R; Emmenegger, T; Fraser, K; Gilg, O; Guerrero, M; Hallworth, M T; Hewson, C; Jiguet, F; Johnson, J A; Kelly, T; Kishkinev, D; Leconte, M; Lislevand, T; Lisovski, S; Lopez, C; McFarland, K P; Marra, P P; Matsuoka, S M; Matyjasiak, P; Meier, C M; Metzger, B; Monros, J S; Neumann, R; Newman, A; Norris, R; Part, T; Pavel, V; Perlut, N; Piha, M; Reneerkens, J; Rimmer, C C; Roberto-Charron, A; Scandolara, C; Sokolova, N; Takenaka, M; Tolkmitt, D; van Oosten, H; Wellbrock, A H J; Wheeler, H; van der Winden, J; Witte, K; Woodworth, B K; Prochazka, P J Anim Ecol, 89 (1), S. 207-220, 2020, ISSN: 0021-8790. @article{RN7c, title = {Weak effects of geolocators on small birds: A meta-analysis controlled for phylogeny and publication bias}, author = {V Brlik and J Kolecek and M Burgess and S Hahn and D Humple and M Krist and J Ouwehand and E L Weiser and P Adamik and J A Alves and D Arlt and S Barisic and D Becker and E J Belda and V Beran and C Both and S P Bravo and M Briedis and B Chutny and D Cikovic and N W Cooper and J S Costa and V R Cueto and T Emmenegger and K Fraser and O Gilg and M Guerrero and M T Hallworth and C Hewson and F Jiguet and J A Johnson and T Kelly and D Kishkinev and M Leconte and T Lislevand and S Lisovski and C Lopez and K P McFarland and P P Marra and S M Matsuoka and P Matyjasiak and C M Meier and B Metzger and J S Monros and R Neumann and A Newman and R Norris and T Part and V Pavel and N Perlut and M Piha and J Reneerkens and C C Rimmer and A Roberto-Charron and C Scandolara and N Sokolova and M Takenaka and D Tolkmitt and H van Oosten and A H J Wellbrock and H Wheeler and J van der Winden and K Witte and B K Woodworth and P Prochazka}, doi = {10.1111/1365-2656.12962}, issn = {0021-8790}, year = {2020}, date = {2020-01-01}, journal = {J Anim Ecol}, volume = {89}, number = {1}, pages = {207-220}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Galhano, V; Hartmann, S; Monteiro, M S; Zeumer, R; Mozhayeva, D; Steinhoff, B; Muller, K; Prenzel, K; Kunze, J; Kuhnert, K D; Schonherr, H; Engelhard, C; Schlechtriem, C; Loureiro, S; Soares, Amvm; Witte, K; Lopes, I Aquat Toxicol, 220 , S. 105404, 2020, ISSN: 0166-445x. @article{RN2x, title = {Impact of wastewater-borne nanoparticles of silver and titanium dioxide on the swimming behaviour and biochemical markers of Daphnia magna: An integrated approach}, author = {V Galhano and S Hartmann and M S Monteiro and R Zeumer and D Mozhayeva and B Steinhoff and K Muller and K Prenzel and J Kunze and K D Kuhnert and H Schonherr and C Engelhard and C Schlechtriem and S Loureiro and Amvm Soares and K Witte and I Lopes}, doi = {10.1016/j.aquatox.2020.105404}, issn = {0166-445x}, year = {2020}, date = {2020-01-01}, journal = {Aquat Toxicol}, volume = {220}, pages = {105404}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2019 |
Hase, M A; Wellbrock, A H J; Bäuerlein, C; Bäuerlein, K; Kelsey, N A; Witte, Klaudia GPS bringt es auf den Punkt! Erste Aufzeichnungen zu Zug- und Überwinterungsdaten beim Mauersegler mit Hilfe von GPS-Datenspeichern Artikel Vogelwarte, 57 , S. 314-315, 2019. @article{hase2019gps, title = {GPS bringt es auf den Punkt! Erste Aufzeichnungen zu Zug- und Überwinterungsdaten beim Mauersegler mit Hilfe von GPS-Datenspeichern}, author = {M A Hase and A H J Wellbrock and C Bäuerlein and K Bäuerlein and N A Kelsey and Klaudia Witte}, year = {2019}, date = {2019-01-01}, journal = {Vogelwarte}, volume = {57}, pages = {314-315}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Wellbrock, A H J; Kelsey, N A; Heldmaier, G; Rozman, J; Witte, K Mauersegler im Energiesparmodus - Hinweis auf Topror bei freilebenden Mauerseglern durch indirekte Kaloriemetrie Artikel Vogelwarte, 57 (4), S. 263-265, 2019. @article{Wellbrock2019, title = {Mauersegler im Energiesparmodus - Hinweis auf Topror bei freilebenden Mauerseglern durch indirekte Kaloriemetrie}, author = {A H J Wellbrock and N A Kelsey and G Heldmaier and J Rozman and K Witte}, year = {2019}, date = {2019-01-01}, journal = {Vogelwarte}, volume = {57}, number = {4}, pages = {263-265}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Schaub, Tonio; Wellbrock, Arndt HJ; Rozman, Jan; Witte, Klaudia Light data from geolocation reveal patterns of nest visit frequency and suitable conditions for efficient nest site monitoring in Common Swifts Apus apus Artikel Bird Study, 66 (4), S. 519–530, 2019. @article{schaub2019lightb, title = {Light data from geolocation reveal patterns of nest visit frequency and suitable conditions for efficient nest site monitoring in Common Swifts Apus apus}, author = {Tonio Schaub and Arndt HJ Wellbrock and Jan Rozman and Klaudia Witte}, year = {2019}, date = {2019-01-01}, journal = {Bird Study}, volume = {66}, number = {4}, pages = {519--530}, publisher = {Taylor & Francis}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Jiang, X; Bao, H; Merzendorfer, H; Yang, Q Immune Responses of Mammals and Plants to Chitin-Containing Pathogens Artikel Adv Exp Med Biol, 1142 , S. 61-81, 2019, ISSN: 0065-2598 (Print) 0065-2598 (Linking). @article{RN6, title = {Immune Responses of Mammals and Plants to Chitin-Containing Pathogens}, author = {X Jiang and H Bao and H Merzendorfer and Q Yang}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31102242}, doi = {10.1007/978-981-13-7318-3_4}, issn = {0065-2598 (Print) 0065-2598 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {Adv Exp Med Biol}, volume = {1142}, pages = {61-81}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Merzendorfer, Hans Chitosan Derivatives and Grafted Adjuncts with Unique Properties Buchkapitel Cohen, Ephraim; Merzendorfer, Hans (Hrsg.): Extracellular Sugar-Based Biopolymers Matrices, S. 95-151, Springer International Publishing, Cham, 2019, ISBN: 978-3-030-12919-4. @inbook{RN1h, title = {Chitosan Derivatives and Grafted Adjuncts with Unique Properties}, author = {Hans Merzendorfer}, editor = {Ephraim Cohen and Hans Merzendorfer}, url = {https://doi.org/10.1007/978-3-030-12919-4_3}, doi = {10.1007/978-3-030-12919-4_3}, isbn = {978-3-030-12919-4}, year = {2019}, date = {2019-01-01}, booktitle = {Extracellular Sugar-Based Biopolymers Matrices}, pages = {95-151}, publisher = {Springer International Publishing}, address = {Cham}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
Merzendorfer, Hans; Cohen, Ephraim Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications Buchkapitel Cohen, Ephraim; Merzendorfer, Hans (Hrsg.): Extracellular Sugar-Based Biopolymers Matrices, S. 541-624, Springer International Publishing, Cham, 2019, ISBN: 978-3-030-12919-4. @inbook{RN50, title = {Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications}, author = {Hans Merzendorfer and Ephraim Cohen}, editor = {Ephraim Cohen and Hans Merzendorfer}, url = {https://doi.org/10.1007/978-3-030-12919-4_14}, doi = {10.1007/978-3-030-12919-4_14}, isbn = {978-3-030-12919-4}, year = {2019}, date = {2019-01-01}, booktitle = {Extracellular Sugar-Based Biopolymers Matrices}, pages = {541-624}, publisher = {Springer International Publishing}, address = {Cham}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
Muthukrishnan, S; Merzendorfer, H; Arakane, Y; Yang, Q Chitin Organizing and Modifying Enzymes and Proteins Involved In Remodeling of the Insect Cuticle Artikel Adv Exp Med Biol, 1142 , S. 83-114, 2019, ISSN: 0065-2598 (Print) 0065-2598 (Linking). @article{RN5d, title = {Chitin Organizing and Modifying Enzymes and Proteins Involved In Remodeling of the Insect Cuticle}, author = {S Muthukrishnan and H Merzendorfer and Y Arakane and Q Yang}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31102243}, doi = {10.1007/978-981-13-7318-3_5}, issn = {0065-2598 (Print) 0065-2598 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {Adv Exp Med Biol}, volume = {1142}, pages = {83-114}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Steinfeld, L; Vafaei, A; Rosner, J; Merzendorfer, H Chitin Prevalence and Function in Bacteria, Fungi and Protists Artikel Adv Exp Med Biol, 1142 , S. 19-59, 2019, ISSN: 0065-2598 (Print) 0065-2598 (Linking). @article{RN4h, title = {Chitin Prevalence and Function in Bacteria, Fungi and Protists}, author = {L Steinfeld and A Vafaei and J Rosner and H Merzendorfer}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31102241}, doi = {10.1007/978-981-13-7318-3_3}, issn = {0065-2598 (Print) 0065-2598 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {Adv Exp Med Biol}, volume = {1142}, pages = {19-59}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Krauss, S; Evert, B O The Role of MicroRNAs in Spinocerebellar Ataxia Type 3 Artikel J Mol Biol, 431 (9), S. 1729-1742, 2019, ISSN: 1089-8638 (Electronic) 0022-2836 (Linking). @article{RN33b, title = {The Role of MicroRNAs in Spinocerebellar Ataxia Type 3}, author = {S Krauss and B O Evert}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30664869}, doi = {10.1016/j.jmb.2019.01.019}, issn = {1089-8638 (Electronic) 0022-2836 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {J Mol Biol}, volume = {431}, number = {9}, pages = {1729-1742}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Reichenbach, N; Delekate, A; Plescher, M; Schmitt, F; Krauss, S; Blank, N; Halle, A; Petzold, G C Inhibition of Stat3-mediated astrogliosis ameliorates pathology in an Alzheimer's disease model Artikel EMBO Mol Med, 11 (2), 2019, ISSN: 1757-4684 (Electronic) 1757-4676 (Linking). @article{RN30b, title = {Inhibition of Stat3-mediated astrogliosis ameliorates pathology in an Alzheimer's disease model}, author = {N Reichenbach and A Delekate and M Plescher and F Schmitt and S Krauss and N Blank and A Halle and G C Petzold}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30617153}, doi = {10.15252/emmm.201809665}, issn = {1757-4684 (Electronic) 1757-4676 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {EMBO Mol Med}, volume = {11}, number = {2}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Schilling, J; Broemer, M; Atanassov, I; Duernberger, Y; Vorberg, I; Dieterich, C; Dagane, A; Dittmar, G; Wanker, E; van Roon-Mom, W; Winter, J; Krauss, S Deregulated Splicing Is a Major Mechanism of RNA-Induced Toxicity in Huntington's Disease Artikel J Mol Biol, 2019, ISSN: 1089-8638 (Electronic) 0022-2836 (Linking). @article{RN16b, title = {Deregulated Splicing Is a Major Mechanism of RNA-Induced Toxicity in Huntington's Disease}, author = {J Schilling and M Broemer and I Atanassov and Y Duernberger and I Vorberg and C Dieterich and A Dagane and G Dittmar and E Wanker and W van Roon-Mom and J Winter and S Krauss}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30711541}, doi = {10.1016/j.jmb.2019.01.034}, issn = {1089-8638 (Electronic) 0022-2836 (Linking)}, year = {2019}, date = {2019-01-01}, journal = {J Mol Biol}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ghizelini, Angela Michelato; Martins, Kelly Geronazzo; Gießelmann, Urs Christian; Santoro, Erika; Pasqualette, Laura; ç, Leda Mendon C S; Rosado, Alexandre Soares; Macrae, Andrew Fungal communities in oil contaminated mangrove sediments - Who is in the mud? Artikel Marine pollution bulletin, 139 , S. 181–188, 2019. @article{Ghizelini.2019, title = {Fungal communities in oil contaminated mangrove sediments - Who is in the mud?}, author = {Angela Michelato Ghizelini and Kelly Geronazzo Martins and Urs Christian Gießelmann and Erika Santoro and Laura Pasqualette and Leda C S Mendon{ç}a-Hagler and Alexandre Soares Rosado and Andrew Macrae}, doi = {10.1016/j.marpolbul.2018.12.040}, year = {2019}, date = {2019-01-01}, journal = {Marine pollution bulletin}, volume = {139}, pages = {181--188}, abstract = {Mangroves are ecosystems located in tropical and subtropical regions of the world and are vital for coastal protection. Their unique characteristics make them hotspots for carbon cycling and biological diversity. Studies on isolated filamentous fungi and environmental and anthropogenic factors that influence sediments offer new understandings on how to preserve mangroves. Here we report on the filamentous fungi isolated from four mangroves. We correlated fungal community composition with sediment texture, polycyclic aromatic hydrocarbons concentration (oil pollution), pH, salinity, organic matter, total and thermotolerant coliforms (sewage pollution). In total we identified 34 genera and 97 species. The most polluted sites had highest species richness whereas the best preserved site showed the lowest species richness. Oil spill and sewage pollution were identified as the drivers of fungal community composition in the most polluted sites. We found very distinct fungal communities with no textgreater5 species shared between any two mangrove sites.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Mangroves are ecosystems located in tropical and subtropical regions of the world and are vital for coastal protection. Their unique characteristics make them hotspots for carbon cycling and biological diversity. Studies on isolated filamentous fungi and environmental and anthropogenic factors that influence sediments offer new understandings on how to preserve mangroves. Here we report on the filamentous fungi isolated from four mangroves. We correlated fungal community composition with sediment texture, polycyclic aromatic hydrocarbons concentration (oil pollution), pH, salinity, organic matter, total and thermotolerant coliforms (sewage pollution). In total we identified 34 genera and 97 species. The most polluted sites had highest species richness whereas the best preserved site showed the lowest species richness. Oil spill and sewage pollution were identified as the drivers of fungal community composition in the most polluted sites. We found very distinct fungal communities with no textgreater5 species shared between any two mangrove sites. |
Gießelmann, Urs Christian; Borchard, Nils; Traunspurger, Walter; Witte, Klaudia European Journal of Soil Biology, 93 , S. 103095, 2019, ISSN: 11645563. @article{Gieelmann.2019, title = {Long-term effects of charcoal on nematodes and other soil meso- and microfaunal groups at historical kiln-sites -- a pilot study}, author = {Urs Christian Gießelmann and Nils Borchard and Walter Traunspurger and Klaudia Witte}, doi = {10.1016/j.ejsobi.2019.103095}, issn = {11645563}, year = {2019}, date = {2019-01-01}, journal = {European Journal of Soil Biology}, volume = {93}, pages = {103095}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fuss, T; Nobel, S; Witte, K J Fish Biol, 94 (5), S. 759-771, 2019, ISSN: 0022-1112. @article{RN6c, title = {It's in the eye of the beholder: visual lateralisation in response to the social environment in poeciliids}, author = {T Fuss and S Nobel and K Witte}, doi = {10.1111/jfb.13953}, issn = {0022-1112}, year = {2019}, date = {2019-01-01}, journal = {J Fish Biol}, volume = {94}, number = {5}, pages = {759-771}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
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