2022 |
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| 1. | Zhu, Weixing; Duan, Yanwei; Chen, Jiqiang; Merzendorfer, Hans; Zou, Xu; Yang, Qing SERCA interacts with chitin synthase and participates in cuticular chitin biogenesis in Drosophila Journal Article Insect Biochemistry and Molecular Biology, 145 , pp. 103783, 2022, ISSN: 0965-1748. Abstract | Links | BibTeX | Tags: Chitin synthesis, Serca @article{ZHU2022103783, title = {SERCA interacts with chitin synthase and participates in cuticular chitin biogenesis in Drosophila}, author = {Weixing Zhu and Yanwei Duan and Jiqiang Chen and Hans Merzendorfer and Xu Zou and Qing Yang}, url = {https://www.sciencedirect.com/science/article/pii/S0965174822000650}, doi = {https://doi.org/10.1016/j.ibmb.2022.103783}, issn = {0965-1748}, year = {2022}, date = {2022-01-01}, journal = {Insect Biochemistry and Molecular Biology}, volume = {145}, pages = {103783}, abstract = {The biogenesis of chitin, a major structural polysaccharide found in the cuticle and peritrophic matrix, is crucial for insect growth and development. Chitin synthase, a membrane-integral β-glycosyltransferase, has been identified as the core of the chitin biogenesis machinery. However, a yet unknown number of auxiliary proteins appear to assist in chitin biosynthesis, whose precise function remains elusive. Here, we identified a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), in the fruit fly Drosophila melanogaster, as a chitin biogenesis-associated protein. The physical interaction between DmSERCA and epidermal chitin synthase (Krotzkopf verkehrt, Kkv) was demonstrated and analyzed using split-ubiquitin membrane yeast two-hybrid, bimolecular fluorescent complementation, pull-down, and immunoprecipitation assays. The interaction involves N-terminal regions (aa 48–81 and aa 247–33) and C-terminal regions (aa 743–783 and aa 824–859) of DmSERCA and two N-terminal regions (aa 121–179 and aa 369–539) of Kkv, all of which are predicted be transmembrane helices. While tissue-specific knock-down of DmSERCA in the epidermis caused larval and pupal lethality, the knock-down of DmSERCA in wings resulted in smaller and crinkled wings, a significant decrease in chitin deposition, and the loss of chitin lamellar structure. Although DmSERCA is well-known for its role in muscular contraction, this study reveals a novel role in chitin synthesis, contributing to our knowledge on the machinery of chitin biogenesis.}, keywords = {Chitin synthesis, Serca}, pubstate = {published}, tppubtype = {article} } The biogenesis of chitin, a major structural polysaccharide found in the cuticle and peritrophic matrix, is crucial for insect growth and development. Chitin synthase, a membrane-integral β-glycosyltransferase, has been identified as the core of the chitin biogenesis machinery. However, a yet unknown number of auxiliary proteins appear to assist in chitin biosynthesis, whose precise function remains elusive. Here, we identified a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), in the fruit fly Drosophila melanogaster, as a chitin biogenesis-associated protein. The physical interaction between DmSERCA and epidermal chitin synthase (Krotzkopf verkehrt, Kkv) was demonstrated and analyzed using split-ubiquitin membrane yeast two-hybrid, bimolecular fluorescent complementation, pull-down, and immunoprecipitation assays. The interaction involves N-terminal regions (aa 48–81 and aa 247–33) and C-terminal regions (aa 743–783 and aa 824–859) of DmSERCA and two N-terminal regions (aa 121–179 and aa 369–539) of Kkv, all of which are predicted be transmembrane helices. While tissue-specific knock-down of DmSERCA in the epidermis caused larval and pupal lethality, the knock-down of DmSERCA in wings resulted in smaller and crinkled wings, a significant decrease in chitin deposition, and the loss of chitin lamellar structure. Although DmSERCA is well-known for its role in muscular contraction, this study reveals a novel role in chitin synthesis, contributing to our knowledge on the machinery of chitin biogenesis. |